Composite

Part:BBa_K892011

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-10-01)

sfGFP-osmY


osmY fused to superfolder GFP with a serine-glycine linker, with sfGFP upstream of osmY. This part was made by swapping out the mamI coding sequence from BBa_K590016 and replacing it with BBa_K892008 using Gibson assembly. This part was characterized using the method outlined in the [http://2012.igem.org/Team:Washington/Protocols/osmY_Assay Washington 2012 wiki], the data summary is shown below.

Each histogram depicts the average normalized fluorescence values for cells plus supernatant, cells only, and supernatant only. The image in the center is a visual representation of the presented data.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 94


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Categories
Parameters
n/asfGFP-osmY